Introduction: Diabetes is a serious problem and has an impact on female sexual function. Aim: The aim of this work was to asses the prevalence of sexual dysfunction among married diabetic females more than 10 years of duration.Methods: This study was carried on 350 married women, age range 30-60 years old, women were divided into two groups 250 diabetic patients from outpatients clinics of National Institute of Diabetes and Endocrine Diseases in Kasr El Aini Hospital and Wadi El Neel Hospital and accepted to share in this study and 100 control group non diabetic women. Main outcome measures: After history taking from all women, blood pressure was measured then laboratory investigations which includes fasting blood sugar, postprandial and glycosylated hemoglobin to the diabetic group. We used the female sexual function index (FSFI),a recognized 19-item questionnaire to assessment of female sexual function for both groups.Results: The result of current study summarized as following: there was a significant decrease in score of total FSF score and all domain that include desire, arousal, orgasm and vaginal lubrication in comparison between diabetic and control women. Diabetics more than 15-year duration were found to have decrease in all domains of FSFI compared with those having duration less than 15 years. There were no significant differences between type I and type II of diabetes with respect to FSFI scoring and FSD percentage. There was statistically significance inverse relation between FSFI and FBS & PP. there was no significance with Hb1AC. FSD was significantly greater in hypertensives than in non-hypertensives. Conclusion: Diabetes mellitus significantly affects female sexual function