Copper and iron are essential elements, the liver is the principle target for copper and iron toxicity. However, relationships between chronic liver diseases and trace elements have not yet clearly understood.The aim of the study is to evaluate role of both copper and iron in the pathogenesis of chronic liver diseases.Forty five cholestatic patients (mean age 3.2m+3.19, ranging between 1-22 months), 37 chronic viral hepatitis C patients (mean age 9.15 y + 4y, ranging between 3-18 years) and 28 healthy controls were included in the study.They were subjected to laboratory investigations (complete blood picture, liver function tests), ultrasonographic assessment, histopathological assessment of needle liver biopsies, assessment of serum iron, TIBC, serum ferritin, serum ceruloplasmin. Serum and tissue copper in liver biopsies using Atomic Absorption Apparatus. Our results showed, that patients with chronic viral hepatitis C had high level of iron stores with subsequent hepatic fibrosis. Serum ferritin was a good predictor for tissue iron overload. Cholestatic patients had elevated levels of serum and tissue copper compared to controls.