Osteoporosis is a major global health problem affects mainly elderly and postmenopausal with increased risk of bone fracture. In an attempt to evaluate different biochemical markers of bone turnover as a diagnostic tool for osteoporosis in postmenopausal women, 40 postmenopausal females, diagnosed by DEXA as osteoportotic were investigated against 24 healthy controls and 16 perimenopausal non-osteoporotic females. All groups were subjected to assessment of fasting urinary calcium (Uca), total ALP, inorganic phosphorous (Pi), urinary deoxypyridinoline (DPD), carboxyterminal telopepiide of collagen type I ICTP and serum N-terminal telopeptide (NTx). The postmenopasual osteoporotic group showed significantly higher levels of ALP, DPD, NTx and ICW compared to controls and only ALP was significantly higher in perimenopausal group compared to controls. However, there was no significant difference of Uca nor Pi among the three groups. On comparing pen and postmenopausal groups together, DPD, NTx and ICTP were the only markers that clearly discriminated between the two groups, being higher in the osteoporotic postmenopausal group. There were significant negative correlations between BMD and each of ALP, DPD, NTx and ICTP. Meanwhile, non significant correlations were found between age and all of the studied markers. We concluded that urinary DPD and serum NTx were excellent non invasive biochemical markers of bone resorption which at cut off levels of 6.8 nm DPD/nM creatinine and 17.0 nM BCE respectively, their diagnostic sensitivity were 100% for both and specificity were 100% for DPD and 98% for NTx. ICTP had a lower specificity (65%), at cut off level 3.6 Ug/L, while its sensitivity was 88% as evidenced by ROC curves analysis.