Back ground: Melatonin is a widely occurring neurotransmitter-like compound derivedprimarily from the pineal gland. Studies have suggested that a relationship exists among sleep,pineal function, and melatonin levels.Aim of study: The aim of this study is to detect the possible melatonin alteration in epilepticpatients.Patients and methods: This study was done on 66 Subjects; 46 patients and 20 controls thatwere matched regarding age, sex and pubertal stages. All subjects in this study were subjected toSleep Disturbance Scale for Children questionnaire, a self-administered rating scale for pubertaldevelopment and 12 hours urinary 6-hydroxymelatonin sulfate measurement.Conclusion: Urinary 6 hydroxymelatonin sulfate level was lower in epileptic patients comparedto the control group and the type of epilepsy and whether it is refractory or not did not affectmelatonin level.