Sewage treatment process involves various hazardous exposures, among which are the polycyclic aromatic hydrocarbons (PAHs) that sewage system receives from mainly industrial sources. Our study aimed at assessing PAHs exposure and verifying the susceptibility to oxidative DNA damage and genotoxicity among sewage treatment workers. The entire exposed group (n=50) and matched control (n=50) were subjected to estimation of 1-hydroxypyrene (1-OHP) and 8-hydroxy-2ˋ-deoxyguanosine (8-OHdG) in urine as biomarkers of exposure and response respectively in addition to environmental assessment of air levels for 16 PAHs. The study revealed significant increase in urinary (1-OHP) and (8-OHdG) in the exposed group compared to their control. Furthermore, the study pointed to the negative impact of PAHs as a cause of oxidative DNA damage and genotoxicity.