Background The rising incidence of Acinetobacter infection in the ICU and in patients with immature or defective body defense system cause a great concern to all clinicians worldwide due to their extraordinary ability to develop resistance to multiple classes of antibiotics which limit array of the therapeutic options. Objective Is to determine the prevalence of Acinetobacter infection in ICUs of Kasr EL-Aini Hospital, its demographic features, speciation and antibiotic sensitivity pattern. Methods Samples collected from infected patients in ICUs were subjected to direct microscopic examination and culture on blood, MacConkey and Herellea media. Microbact (12A) Gram-negative identification system was used. Susceptibility patterns were done by Modified Kirby Bauer disc-diffusion methods. Results Out of 140 inpatients suffering from infections, 21.4 % (no=30) were found to be infected with Acinetobacter spp. It was responsible for 26.3% of LRTIs, 20% of wound infections and 16.2% of UTIs. A. baumannii was the most predominant species (93.3%). Prolonged stay in ICU (p=0.03) and stroke (p=0.005) were significantly associated with Acinetobacter infections. Other risk factors include previous antibiotic treatment (OR=1.07). The most effective antibiotics were cefoperazone/sulbactam (40%), imipinem (36.7%) and amikacin (30%). Totally 43.3% (13/30 isolates) were found to be MDR Acinetobacter isolates.Conclusions Infection due to A.baumannii has become a significant challenge to healthcare systems. 21.4% of the studied patients suffered from Acinetobacter infections. Invasive procedures, prolonged stay in ICU as well as previous antibiotic treatment are associated with higher rate of infection. Eradication of Acinetobacter spp. requires adherence to good infection control practices and prudent antibiotic use.