Objective: To assess the association between VDR Bsm I (G/A) genepolymorphism and hepatocellular carcinoma in Egyptian patients. Alsodetermination of serum vitamin D concentration in relation to differentgenotypes and in relation to disease activity.Methods: 45 HCC patients and 43 healthy subjects were subjected toroutine laboratory investigations including AST, ALT and serum hepatitismarkers. Both groups were tested for VDR Bsm (G/A) genotypes byReal-Time PCR and serum 25(OH) vitamin D by ELISA.Results: No significant differences in VDR Bsm (G/A) genotypes oralleles frequencies could be identified between HCC cases and controls(P = 0.974, 0.837 respectively). Also, there was no significant differencecould be identified between VDR Bsm (G/A) genotypes and clinical orlaboratory parameters of HCC except for AST that was significantbetween GG& GA (P = 0.009) and GG & AA (P =0.038). on the otherhand, the median of serum 25(OH) vitamin D concentration wassignificantly lower in HCC cases (2ng/ml) than controls (25ng/ml) (P =0.000). Also, there was a significant association between median 25 (OH)D concentration and diabetes in HCC patients where it was lower in nondiabetic (1.75ng/ml) than diabetic HCC patients (3ng/ml).Conclusion: The results of this study suggest that, VDR Bsm (G/A)gene polymorphisms does not exhibit a significant influence on HCCsusceptibility in Egyptian patients. Nevertheless, serum 25(OH) vitaminD could be a marker for HCC.