Patient safety is a new healthcare discipline that emphasizes the reporting, analysis, and prevention of medical error that often leads to adverse healthcare events. The aim of this study was to assess the culture of patient safety in primary health care in Kuwait. A descriptive cross sectional study was conducted in four primary health care centers in Kuwait over a period of three months using adapted questionnaire of Agency for Healthcare Research and Quality (AHRQ) adapted questionnaire. Two hundred and seventy six (276) hospital staff were included in this study. The survey measured fourteen (14) dimensions related to the aspects of safety culture through 44 items in addition to seven (7) items as background variables related to the staff demographics. The study results revealed six safety dimensions with lowest positivity and need to be considered of high priority focused areas. These dimensions are the Non - punitive response to errors, Frequency of event reporting, Staffing, Communication Openness, Hospital Handoffs and Transitions and Supervisor/manager expectations and actions promoting safety with the following percentages of positivity 24%, 32%, 41%, 45%, 47% and 53% respectively. So, improving patient safety culture should be a priority among health center administrators. This can be achieved through modifying an event reporting system and encouraging personnel to report probable errors and events and provide safety education to front- line staff, managers, and physicians that includes team training and education in communication techniques.