Introduction: C-kit positive cells outside the gastrointestinal tract(GIT) which are morphologically similar to interstitial cells of cajal (ICC)in the GIT were supposed to play same role of motility in organs otherthan the GIT. Aim of the work: To observe the effect of ischemiareperfusion injury I/R injury on the rat vas deferens with reference tointerstitial cajal like cells (ICLC). Materials and Methods: This studywas performed on 35 male albino rats weighing 180-200 gm divided into3 main groups; control group (A), ischemia group (B) and reperfusiongroup (C) after applying spermatic cord torsion for one hour followed bydetorsion. All sections from the different groups were stained withheamatoxylin and eosin (H & E), toludine blue and c-kitimmunohistochemichal staining and morphometric study was donerecording the mean area percent of c-kit positive (ICLC). Results: Theexamined H & E sections showed histological changes in ischemia groupand the first two subgroups of reperfusion C1, C2 and relative recovery inthe other two subgroups C3, C4. The mean area percentage of c-kitpositive ICLC in the ischemia group and the first two subgroups C1, C2of reperfusion was significantly decreased compared to the control. Theother two subgroups C3, C4 of the reperfusion group showed relativelyincreasing of the mean area percentage of the c-kit positive ICLCcompared to the control. Conclusions: it was concluded that I/R injuryhad evident histopathologicl effects on the vas deferens that affected alsoits ICLC. This changes might explain abnormal vas deferens motility inI/R injury and might explain some of infertility causes.