Introduction: Exploring the hypothesis that “sexual function” is associated with mode of delivery is important, because sexual health is an integral part of general health. Aim: The aim of this study was to evaluate the relationship between mode of delivery and subsequent incidence of sexual dysfunction and impairment of sexual quality of life (SQoL) in women. Main Outcome Measures: Sexual function as well as sexual quality of life of females were assessed using Female Sexual Function Index (FSFI) and sexual quality of life – female (SQoL-F). Methods: A total of 100 women (mean age 29.1 ± 3.11 years, range 25–35 years) were recruited in this cross sectional study. Females were divided into two groups according to their mode of delivery, including: group A, normal labour (NL) (group NVD, N = 45); group B, cesarean section (C/S) (group C/S, N = 55). Results: The mean period for resumption of sexual intercourse 1st intercourse after the 1st labour was 6.1±1.92 weeks. There was significant difference in mean score of FSFI before and after C/S (P = 0.04), while no difference was observed in NL (P = 0.07). Desire domain score showed significant difference between pre and post 1st labour for both NL ( P = 0.001)and C/S( P = 0.01) while orgasm, satisfaction and pain domains showed no significant difference. Arousal and lubrication domains scores showed significant difference between pre and post 1st labour in NL where P equals 0.01 and 0.03 respectively, while they showed no significant difference in C/S. The research showed no significant difference in standardized score of SQoL-F between NL and C/S groups. The most important significant factor for prediction of SQoL-F score was assistance in house work. Conclusions: NL is associated with the higher rate sexual dysfunction in comparison with C/S in contrast to SQoL-F which is not similarly affected.