Hepatitis G virus is a human RNA flavivirus distantly related to HCV. Multiple evidences suggest that HGV is a blood born virus transmitted through repeated percutaneous exposure to infectious blood and its products. Patients on maintenance hemodialysis represent a high risk group of parenterally transmitted viral hepatitis infections, such as hepatitis B, D, C and G due to impairment of immune response, blood transfusion and nosocomial transmission in hemodialysis units.The objectives of this study were to determine the prevalence of HGV infection in hemodialysis patients, comparing its prevalence to the seroprevalence of other parenterally transmitted viral hepatitis and to find out any possible association between HGV and those viruses in hemodialysis patients. The study was conducted on 81 individuals, 71 hemodialysis patients, 43 males and 28 females. A group of 10 normal healthy individuals were selected as a control group. The latter group had no history of blood transfusion, dental visits and operations in the previous six months. They were also selected to be with normal liver and kidney functions and seronegative for HBsAg, anti-HCV and anti-HIV (1,2) by ELISA technique. The former group which included the 71 hemodialysis patients had their sera been tested for liver and kidney functions. They were also tested by ELISA technique for HBsAg, anti-HCV and anti-HIV (1,2). All patients’ sera were tested for GBV-C/HGV-RNA by RT-PCR. The prevalence of GBV-C/HGV-RNA in hemodialysis patients was found to be 17% with prevalence of HCV in HGV positive hemodialysis patients higher (83.3%) than the prevalence of HBV (6.3%).In spite of that AST and ALP enzymes reported significant difference between the positive hepatitis cases and the control group; yet, no significant statistical difference in ALT, AST and ALP was reported between hemodialysis patients with single HCV infection and those with combined HCV and HGV. Analysis of risk factors revealed that blood transfusion was reported in 8 out of 12 HGV positive cases (66.6%) (p value<0.05) and combined HCV infection was in 10 out of 12 HGV positive cases (83.3%) (p value<0.01). Also there was a highly significant correlation between duration of renal dialysis and positive detection of GBV-C/HGV-RNA in serum. This shows that HCV, blood transfusion and duration of dialysis were risk factors significantly associated with increased risk of GBV-C/HGV-RNA positivity. On the other hand GBV-C/HGV-RNA positivity showed no predilection to certain sex or age group.