Bronchial asthma (BA) is a chronic disorder of the airways. 5-Lipoxygenase (5-LO) play an essential role in the biosynthesis of leukotrienes that induce proinflammatory actions. The aim of this study was to determine the 5-lipoxygenase (5-LO) pathway polymorphisms in asthmatic patients and to understand their role in the pathophysiology of BA. Subjects and Methods: 50 asthmatic patients and 30 controls were included in this study. 3 genes were studied (LTC4S-444A/C using polymerase chain reaction/Restriction Fragment length Polymorphism (PCR/RFLP), ALOX5-176/-147 using fragment length analysis and ALOX5AP-169/-146 by real time PCR).Results: there was statistically significant increase in the mutant genotype of ALOX5AP–169–146 gene among the patients group compared to the control group (P=0.004) with 4.5 times risk of bronchial asthma among those carrying the mutant ‘357’ allele of Alox5AP–169–146 gene (OR= 4.529, 95%CI= 1.7-12.018, P<0.001). ALOX5-176/-147 mutant genotype was higher in patients groups compared to control group though not reaching statistical significant differences (P=0.061) with12.42 times risk of bronchial asthma among those carrying the mutant ‘m’ allele of Alox5–147–176 gene (OR=12.4, 95%CI= 1.548- 99.8,P=0.001). LTC4S–444A/C gene showed no statistical significant differences between the study groups (P=0.679).Conclusion: The homozygous 357/357 genotype of ALOX5AP–169–146 gene was associated with susceptibility to bronchial asthma. No association detected between LTC4s –444A/C and of ALOX5–147–176 genes polymorphisms and susceptibility to asthma.