Streptococcus agalactiae (GBS) is a leading cause of infection in newborns and adults. This study evaluates the difference in efficacy between three different methods for detecting GBS carriage in the vagina of 100 normal healthy women, and tested the susceptibility of the isolates to erythromycin. The vaginal swabs were cultured onto sheep blood agar plate (SBAP). SBAP supplemented with colistin and gentamycin and the swabs were also cultured on selective broth and subcultured from the broth onto SBAP. Erythromycin susceptibility testing of all GBS isolates was done with the E-test. The rate of GBS isolation was 13%, 11% and 10% for culture on selective broth, SBAP with colistin and gentamycin and SBAP respectively. Seven isolates were sensitive to erythromycin.