This study aimed to evaluate the efficacy of detection of Human Chorionic Gonadotropin (beta-HCG in vaginal fluid as a reference in cases complaining of premature rupture of membranes (PROM). Patients and methods: this study included 100 patients which where divided into 2 groups, study group (50 patients) and control group (50 patients). All patients were subjected to detailed history taking, general and local examination, ultrasonographic evaluation of amniotic fluid index (AFI) and speculum examination and sampling. Results: this study showed that β-HCG is a good reliable test in detecting cases of PROM Conclusion: The results showed that β-HCG is highly significant statistical test in detecting cases of PROM and it's more accurate and reliable than AFI in detecting the cases of PROM.