Background: Bullous pemphigoid (BP) is a subepidermal autoimmune skin dissease characterized by tense blisters on normal or erythmatous skin, deposition of IgG at basement membrane zone (BMZ), and circulating IgG against BMZ.Objective: The aim of this study was to determine whether the blister fluid can be used instead of serum for diagnosis of BP.Methods: IIF technique was used for determination of IgG.ELISA technique was used for quantification of IL-6 and TNF-alpha. Both techniques were performed on sera and blister fluids of 30 BP patients compared with sera of 10 normal control subjects.Results:IgG titre in blister fluids and sera of BP patients was the same. The mean of IL-6 levels in blister fluids and sera of BP patients were matched and higher than those in control sera but the mean of TNF-alpha levels in both media were not matched.Conclusion: Our findings demonstrate that the blister fluid can be used instead of serum for detecting BMZ IgG AAbs by IIF technique and quantifying IL-6 and not TNF-alpha by ELISA technique for diagnosis of BP.