The present work aimed at investigating the possible therapeutic effect of garlic extract (GE) on induced wound in non-diabetic and diabetic male albino rat. The possible role of endogenous stem cells (SCs) was also determined. Thirty two adult male albino rats were divided into, Group I (Control group) of 8 rats, Group II (Non-diabetic wounded group) and Group III (Non-diabetic wounded and GE treated group), 6 rats each were exposed to wound induction, in Group III 0.5 ml of 1% GE was locally applied on the wound and on the surrounding skin margins for 6 days/week at an interval of 24 h following wound induction. Group IV (Diabetic wounded group) and Group V (Diabetic wounded and GE treated group), 6 rats each that received single IP injection of STZ 50 mg/kg dissolved in 0.5 ml citrate buffer then exposed to wound induction and in Group V 0.5 ml of 1% GE was locally applied. Each group was subdivided into subgroups a and b, in each 3 animals were sacrificed 2 and 3weeks respectively. Skin sections including the wound and surrounding skin margins were subjected to histological, immunohistochemical, morphometric and statistical studies: In subgroup IIa, separation of the wound edges, epidermal thickening, diffuse eosinophilic homogenous material extending, detached fibers of the horny layer, impacted keratin between the epidermal layers and nuclear margination in multiple vacuolated epidermal cells were noticed. Separation of the C.T fibers, some blood vessels, multiple extravasated RBCs and eosinophilic homogeneous material were observed among the dermis. Less changes and cellular debris in occasional wide vacuolated areas were detected in subgroup IIb. In subgroup IIIa, localized separation of epidermal edges, localized homogeneous material in epidermis, deeply acidophilic material at the surface in addition to epidermal thickening at one side of the wound were observed. Discontinuity of granular and horny cell layers, occasional impacted keratin whorls, few vacuolated cells with nuclear margination and occasional localized vacuolated areas containing cellular debris were seen. Some deep hair follicles, related sebaceous glands and separation of some C.T fibers were noticed. In subgroup IIIb, focal separation of epidermal edges, microfollicles and multiple deep follicles were detected. Few epidermal cells demonstrated nuclear margination. In subgroup IVa, disrupted detached parts of the epidermis and detached parts of the horny layer, homogeneous material containing dark nuclei, multiple congested vessels, multiple extravasated RBCs, mononuclear infiltrating cells and separated CT fibers were seen in the dermis. In subgroup IVb, detached parts of horny layer, widely separated edges, thickening of the epidermis and multiple congested vessels were found. Massive eosinophilic homogeneous material contained multiple dark nuclei and cytoplasmic remnants surrounded by clear spaces. Vacuolated areas among disorganized cells at the edge of the wound and wide separation of the C.T fibers was noticed. In subgroup Va, less changes were observed. Few small hair follicles were detected near the creeping epidermal edges. Occasional deeper follicles and occasional sebaceous glands were demonstrated. Multiple fibroblasts, minimal separation of collagen fibers and some collagen fibers overlied by fibroblasts were seen. In subgroup Vb, less obvious changes, few sebaceous glands, occasional follicles, minimal separation of the CT fibers and few collagen fibers overlied by fibroblasts were found. In control rats few Caspase3 +ve cells, in group II multiple +ve cells, in subgroup IIIa some +ve, in subgroup IIIb few +ve cells, in subgroup IVa multiple +ve cells, in subgroup IVb numerous +ve cells, in subgroup Va some +ve cells, in subgroup Vb fewer +ve cells were found. In subgroup IIa some CD44+ve cells in the epidermis and multiple +ve cells in the dermis, in subgroup IIb, fewer +ve cells, in subgroup IIIa multiple +ve cells in the epidermis, dermis and hypodermis, in subgroup IIIb fewer +ve cells were seen. While in subgroup IVb, more numerous +ve cells were found compared to subgroup IVa. In subgroup Va, some +ve cells in the epidermis, multiple +ve cells in the dermis and in subgroup Vb fewer +ve cells were obvious. The mean distance between epidermal margins showed a significant increase in subgroup IVb compared to other subgroups, in subgroup IVa compared to subgroup IIb and in groups III and V, in subgroup IIa compared to groupIII and in subgroup IIIa compared to subgroup IIIb. A significant increase was found in the mean thickness of the epidermis in subgroups IIa and IVb, compared to control, subgroups IIIb, IVa, Va and Vb. A significant increase was recorded in the mean area of separation between the CT fibers in subgroup IVb compared to subgroup IIIa and group V. A significant increase in the mean area% of caspase3 +ve cells was reported in subgroup IVb compared to control and the other subgroups, in subgroup IVa compared to control, groups III and V and in group II compared to control and groups III and V. A significant increase in the mean area% of CD44 +ve cells was found in subgroup IIIa compared to groups II, IV and V, in subgroup IIIb a significant increase was detected compared to groups II, IV and subgroup Vb and in subgroup Va compared to subgroups IIb and group IV. In conclusion, GE constituting allicin as active constituent proved amelioration of the inflammatory, degenerative, necrotic and apoptotic changes developing at the wound site during healing in nondiabetic and diabetic rats. This therapeutic effect was proved to be related to both caspase3 pathway inactivation and MSC migration activation. This suggests GE or its active constituent, allicin to be applied in appropriate concentrations in the preparation of pharmaceutical products to be used safely on epithelial cells.