Background: Vitamin A is required for normal functioning of the visual system, and maintenance of cell function for growth, epithelial integrity, red blood cell production, immunity and reproduction, its deficiency is considered of one of the major public health problems but also toxicity from vitamin A witch occurs after consuming large amounts of vitamin A over a short period of time, typically within a few hours or days or when large amounts of vitamin A build up in the body over a long period of time is considered hepatotoxic and because of the long half-life of vitamin A in the liver (50 days to 1 year),the fibrotic process may continue due to the slow release of hepatic vitamin A stores despite discontinuation of oral intake of the vitamin. Objective: To assess the level of vitamin A status in infants at 9 months of age who were not supplemented by vitamin A, and in infants at 9 months who were supplemented with 100,000 IU of vitamin A. Methods: A hundred healthy breast fed infants were enrolled in this cross-sectional study, 50 of them received a compulsory doses of 100.000 IU of vitamin A.We assessed growth by anthropometric measurements and clinical examination. We assessed retinol level by high frequency liquid chromatography. Results: The mean of serum retinol was 53.18±8.74 in the non -supplemented group and a mean of 59.78±7.07 supplemented group (p value=0.00) which is all within the normal range (11.3 and 64.7 mcg/dL). The mean of serum AST, ALT and albumin in un-supplemented group was 4.83±2.57, 7.83±2.81 and 3.55±0.41 respectively while the mean of serum AST, ALT and albumin in supplemented group was 6.37±2.28, 19.96±3.42 and 3.91±0.46 respectively and There was a positive statistically correlation between serum retinol level and liver functions (AST, ALT and albumin) between both groups (p value =0.000, 0.002, 0.000, 0.0000) respectively. All values are also within the normal range of liver function. There was no correlation between the serum retinol level and the sex, weight and height of the studied groups (p value =1.000, 0.672, 0.338) respectively. Conclusion: This study showed that level of serum retinol was within normal range before and after the vitamin A supplementation in infants at nine months of age. The study found the need for vitamin A at 9 months is questionable, yet the sample size and its confinement to a small geographic area that is not representative of Egyptian variance call for more prospective trials to define timing of decline of serum retinol and support need for its supplementation and time of supplementation if needed.