This work is a case control study that is carried out to determine the relation between colorectal cancer in one side and socio-demographic, environmental risk factors, dietary intake of folate and B12 in other sides among 212 cases recruited from National Cancer Institute and 212 control free of any diseases. Cases and controls were submitted to the following steps of work interviewed to fulfill a questionnaire about risk factors of colorectal cancer (socio-demographic and environmental risk factors), then 100 cases and their matched controls wee asked for items of food frequency sheet, A sub-sample of 50 cases and their matched controls (taken from 100 cases and controls who filled food frequency sheet), serum folate and megaloblastic anemia were measured.Results : The lower intake of folate and B12 is more common with colorectal cancer patients . There is strong correlation between colorectal cancer and low serum folate and B12. There are important risk factors that may contributed to cancer colon such as (rural residence, exposures to chemicals such as car exhaustion, motor oils, diagnostic radiation, history of spastic colon, history of bilharzias, using kerosene stove for long duration).It is recommended that folic acid can play an important role in the prevention of colorectal cancer. There is a great need to put a health education strategy aiming to prevent colorectal cancer.