The present work aims at defining the male factors affecting the blastocyst formation in terms of number, quality and implantation rate in couples undergoing ICSI when fresh epididymal (group 2: n= 17) and testicular (obstructive (group 3: n= 13) and non-obstructive(group 4: n= 21)) sperms were used and to compare these data to the results of intracytoplasmic sperm injection of ejaculated sperms (group 1: n= 21) taking in consideration genetic defects including Yq 11 (microdeletions) (group 5: n= 9), CFTR variants and mutations (congenital bilateral absence of the vas deferens) (group 6: n= 13) and topographic site of TESE (testicular sperm extraction). In the present study, the outcome of ICSI treatment using spermatozoa from men with a microdeletion of the Y chromosome was compared with that of ICSI treatment using spermatozoa from oligozoospermic men without these deletions. Clinical pregnancy rate per cycle, implantation rate per embryo transferred and multiple pregnancy rate were 22.2% versus 42.9%, 27.8% versus 35% and 50% versus 55.5% respectively.Fertilization and blastocyst formation rates were significantly lower in group 4 (P < 0.05). The incidence of expanded and hatching blastocysts was significantly lower in group 4 (P < 0.05). Overall in 93% ejaculate ICSI cycles, blastocysts were transferred on day 5. This was significantly higher than the 55.6% day 5 transfers in the non-obstructive azoospermic group (P < 0.05). Implantation rate per embryo was significantly higher in the ejaculate ICSI group compared with the other groups (P < 0.05). Clinical pregnancy per transfer was similar between groups; however, significantly fewer multiple pregnancies were encountered in the non-obstructive azoospermic group (P < 0.01).AF508 genotypes demonstrated a satisfactory fertilization rate, 2PN fertilization rate, cleavage rate, blastogenesis rate, clinical pregnancy rate, implantation rate embryo and multiple pregnancy rate when compared with those of the ejaculate group. So, we concluded that, cystic fibrosis mutations in the male partner do not appear to compromise oocyte fertilization, embryo implantation rates, or the opportunity for blastocyst stage development and transfer. In conclusion, the site and the source of sperm retrieval, most likely to be like a mirror that highly reflect of the severity of spermatogenic disorder, affects the rate and quality of blastogenesis.