In recent years, there has been an increase in infections caused by extended-spectrum β-lactamase (ESBL)-producing strains and AmpC producing strains of Gram-negative bacilli. These infections are associated with significantly higher morbidity and mortality. Classical microbiology results are not available for at least 48 h after clinical specimens are taken, with this period further extended if additional investigations are done.Chromogenic tests could be used to for rapid detection of ESBL and AmpC producers. In the present study, we aimed to evaluate the Cica-ß-test which uses a chromogenic oxyimino-cephalosporin, HMRZ-86, in combination with various inhibitors, and correlate with standard phenotypic and genotypic techniques. The (30) tested strains comprised clinical isolates of Escherichia coli, Klebsiella spp., Enterobacter spp., Pseudomonas aeruginosa with 6 isolates ESBLs producers, 20 isolates AmpC producers and 4 isolates with other mechanism of resistance ( non ESBL – non AmpC ).The resistance mechanisms had been established previously by standard phenotypic and genotypic methods.The Cica beta test gave 65% to75% agreement with reference data which equate to sensitivity and specificity rates 65.35 and 75% respectively.The use of rapid methods like Cica beta test for early detection and diagnosis of ESBL and AmpC producers may be useful early guide.