Diabetes mellitus type 2 is known as one of the factors causing the cholesterol gallstones. The aim of the present study was to investigate gallbladder function (motility) in diabetic patients type 2 and determine its relation with peripheral and autonomic neuropathy. The study demonstrated that there is a significant increase in fasting gall bladder volume and a significant reduction of the ejection fraction in diabetic patients with peripheral neuropathy and patients with autonomic neuropathy, these changes is considered a risk factors for gall stone disease in those patients.