H. pylori infection is involved in the development of gastric cancer. This study aimed at investigating the relationship between the presence of H.pylori infection, infection density, IL-6 level (serum and tissue IL-6) among gastric cancer patients. Thirty gastric cancer patients and thirty controls are enrolled in the study. H.pylori density was assessed by quantitated culture. Antibiotic sensitivity testing was performed. Serum and tissue IL-6 level was measured using ELISA. H.pylori was found in 100% of cases and controls and its density was higher in cases than in controls. All isolated strains were 100% resistant to metronidazole whereas tetracycline was the most effective antimicrobial agent used. Serum IL-6 was detected in cases and controls. However, tissue IL-6 was detected in gastric biopsies of gastric cancer patients only and was below detectable levels in all controls. Tissue IL-6 could be a good diagnostic marker of stomach cancer.