Umbilical vein catheterization is a procedure that is commonly used in neonatal units. Echocardiography performed for any indication in neonates with umbilical venous catheter reveals a high incidence of catheter malpositioning so it has been suggested that echocardiography should be used as the gold standard to confirm ideal catheter position. To minimize potential complications of incorrect catheter placement, the catheter tip should be at the right atrial inferior vena cava junction or in the thoracic inferior vena cava. The aim of this study was to evaluate and compare the validity of traditional tools such as anteroposterior chest X ray, lateral chest X ray, and umbilical blood gas analysis versus echocardiography for prediction of appropriate catheter location. The study was carried on 50 neonates (27 males, and 23 females) who were indicated for umbilical venous catheter placement. Their mean (SD) post natal age was 4.62 ± 2.03 days. All neonates were subjected to radiological and laboratory investigations that involve anteroposterior chest X ray, lateral chest X ray, umbilical blood gas sampling, and echocardiography with saline contrast injection. Findings revealed that the AP CXR and Lat CXR were similarly sensitive (72.73%) in assessing ideal catheter position (inferior vena cava/right atrial junction) with the AP CXR being more specific (100% versus 87.1%). Moreover, Lat CXR was less sensitive than AP CXR (60% versus 100%) in assessing catheter malposition (left atrial catheter placement) with both having the same specificity (100%). Finally, the oxygenation data were highly sensitive (80%) and specific (91.11%) in assessing catheter tip placement in the left atrium. Thus, it is concluded that echocardiography with saline contrast injection is more accurate in assessing and confirming appropriate umbilical venous catheter location than traditional radiological and laboratory methods.