OCT-4 (also known as POU5F1) and BMI-1 (a member of PRC1) are the key regulators of self-renewal in embryonic stem cells. Regarding the new cancer stem cell concept, the expression of such genes are potentially correlated with tumorigenesis and can affect some aspects of tumor behavior, such as tumor recurrence or resistance to therapies. Although OCT-4 and BMI gene expression has been introduced as a molecular marker for germ cell tumors, little is known about their expression in somatic cancers. Here, we have investigated the potential expression of OCT-4 and BMI in bladder cancer. We used Real Time-PCR (RT-PCR) to examine the expression of OCT-4 and BMI genes in 12 patients recently diagnosed as Urothelial Cell Carcinoma (UCC) , tissues taken from Tumor biopsy. Culture of cells were done in serum free media, some of them were taken for clonogenic assay and the rest for detection of genes expression using RT-PCR. our results showed late expression of BMI Gene before development of advanced staging and grading or lymph node enlargements. OCT 4 Gene showed early expression in contrast to BMI gene expression.