Receptor tyrosine kinases (RTKs) are a group of enzymes involved in a variety of physiological and pathological processes. The human ROR1 is a member of the RTK family with unknown ligand and biological function. Over expression of ROR1 has recently been reported in B-cell chronic lymphocytic leukemia.The aim of this study was to detect the expression profile of ROR1 in acute lymphoblastic leukemia (ALL) cells. Leukemic cells were isolated from the peripheral blood (PB) of 54 ALL children. Thirty age and sex matched healthy volunteers were used as controls. Immunophenotyping was performed by flowcytometry and mRNA expression was detected by semi quantitative RT-PCR.Over expression of ROR1 mRNA was detected in 15 of 54 (27.8%) ALL children, which is statistically higher than ROR1 expression in control groups (3/30, 10%), P. value 0.048.Also, the ROR1/ β-Actin ratio was higher in ALL children, which is statistically higher than ROR1/β-Actin ratio in control group, P. value 0.024. There has been neither a significant association between ROR1 expressions and the immunophenotypic profile of the leukemic cells, nor with other clinical or hematological features of the patients. ROR1 is a new tumor-associated antigen and a potential tool for targeted immunotherapy and monitoring of minimal residual diseases in ALL patients.