Inactivation of the tumor suppressor gene RASSF1A by promoterhypermethylation of CPG islands was frequently encountered in multiple tumorsincluding HCC.Our study investigated the presence of hypermethylated RASSF1Ain sera of HCC patients , HCV chronic carriers and normal healthyindividuals(using the methylation sensitive restriction enzyme mediated real timePCR technique).The study was conducted on 45 HCC patients, 40 HCV chroniccarriers and 40 healthy individuals. We detected hypermethylated RASSF1A insera of (86.7 % , 62.5% and 5% ) with median concentrations ( 60 , 1.65 and 0copy/ μ L ) for the HCC group, HCV group and heathy control group respectively.These findings may allow the use of this marker for early diagnosis of HCCespecially for those under routine survillence programs.