Objectives: Oral rinse method is widely used, and although it does not directly target specific mucosal lesions, it provides a measure of the candidal load and can permit detection of other microbes. The aim of the current study is to evaluate the value of oral rinse solution as a method to detect Candida albicans in diabetic and non diabetic patients wearing complete denture.Materials and Methods: 40 individuals participated in this study. They were divided into three groups. Group 1 included (15) diabetic patients diagnosed with denture sore mouth (DSM), Group 2 included (15) non-diabetic patients diagnosed with DSM. Group 3 (control group): included (10) healthy patients (systemic free) wearing denture free from Candida infection. After recording the clinical examination of patients with denture sore mouth types, oral rinse solution was collected a portion (10 uL) of the oral rinse solution was cultured on Sabouraud’s dextrose agar (SDA) and (90 uL) of the oral rinse solution was used to measure C.albicans antigen by an ELISA technique.Results: ELISA showed higher sensitivity and diagnostic accuracy than culture in detection of oral candida albicans.Conclusion: Oral rinse sample method was found to accurate and easy way for the detection of oral candidal infection.