Objectives: The use of diode laser for biostimulation of bone and periodontal regeneration, the normal scaling and root planing can not stimulating the bone regeneration . The aim of the current study is to evaluate the adjunctive efficacy of Diode laser on periodontal Regeneration in diabetic patient with chronic periodontitis. Materials and Methods: The selected patients were randomly one group split mouth in anterior lower teeth the right side treated with conventional therapy scaling and root planing, and the left side of lower anterior teeth treated with conventional therapy (supragingival scaling and subgingival debridement) and Laser. Biostimulation Diode laser parameters (weave length= 810, average p power=2-4 watt, delivery system is fibro optic). All the study individuals were subjected to comprehensive oral diagnosis using the department oral diagnosis charts. Use standardized technique with radiographic stunt and x-ray holder. Radiographic examination by Direct digital radiograph (Digora) at time of the initial examination to confirm diagnosis.The test group patients were subjected to conventional therapy (supragingival scaling and subgingival debirdement) and Laser. Results: Direct digital radiograph (Digora) show no significant different at base line and post 4 months, minim increase of bone density post 6 months in laser side than scaling. Conclusion: Compared with SRP alone, low level laser therapy (Diode laser 810nm) in combination with SRP further improved bone density at 4 and 6 months and reduced PD at 6 months. Low-level laser therapy application as an adjunct to non-surgical treatment reduced gingival inflammation in patients with diabetic mellitus and chronic periodontitis.