Aim of study: The aim of study is to compare the bond strength of a biocermic-based sealer, a mineral trioxide aggregate-based sealer and an epoxy resin –based sealer to dentine when treated with different irrigation protocols.Material and methods: 90 freshly extracted straight single rooted human teeth, The roots were randomly divided into three main experimental groups according to the final irrigation regimen as follows: Group I (n=30): after complete instrumentation of prepared canals they were irrigated by 10ml of 2.6%(NaOCL) sodium hypochlorite and 5ml of distalled water. Group II (n=30): prepared canals were irrigated by 5ml of 2.6% NaOCL followed by 5ml of 17%(EDTA) then followed by 5ml distilled water for 1 min. Group III (n=30): prepared canals were irrigated by 5ml of 2.6% of NaOCL followed by 5ml of water then 5ml of 2% (CHX) Chlorohxidin. Each group was subdivided into three subgroups according to the sealer material to be used, as followed: -Sub group A (n=10): Biocermic-based sealer (i Root SP).Subgroup B (n=10): Epoxy resin –based sealer cement (AH Plus).Sub group C (n=10): Mineral trioxide aggregate-based sealer (MTA Fillapex).One horizontal section of 2mm thickness, each was cut from middle thirds of each root were sliced to perform push out bond strenght measurement. There was no statistically significant difference between iRoot and AH Plus; both showed the statistically significantly highest mean push-out bond strength values. MTA showed the statistically significantly lowest mean push-out bond strength. It was concluded that iRoot SP and AH Plus sealers perform similarly and to be preferable to MTA Fillapex in term of bond strength.