Objective: The aim of this study was to isolate and characterize human dental pulp stem cells and to induce their in-vitro differentiation into odontoblast and nerve-like cells. Materials and methods: human dental pulp tissues were collected from impacted third molars immediately after extraction. The collected pulp tissues were then enzymatically digested using 0.2% collagenase type II to release the primary total pulp cells. The obtained cells were cultured in DMEM supplemented with 15% FBS and antibiotic mixture. Isolation of STRO-1+ cells was done using immuno-magnetic separation. STRO-1+ HDPSCs were induced to differentiate into odontoblast-like cells using Dexamethasone/vitamin D3 and into nerve-like cells using a neurogenic induction cocktail. Results: STRO-1+ cells were successfully isolated and were induced to differentiate into odontoblast and nerve-like cells, confirmed by immuno-staining. Conclusions: It was found That STRO-1+ HDPSCs are capable of extensive proliferation and multi-lineage differentiation into more specialized cell types.