Objective: to assess the ability of DNA flowcytometery to identify haploid cells in the ejaculate, predict the outcome of testicular sperm extraction and patients with non- obstructive azoospermia. Patients and Methods: this study was conducted on sixty-five male patients suffering from non-obstructive azoopermia. They were recruited from the andrology and STDs outpatient clinic, Kasr El Aini University Hospital and from Adam international clinic from May 1998 till July 2000. History taking and clinical examination were performed according to the infertility sheet they were also subjected to routine investigations (semen analysis, FSH assay). Testicular volume was determined using Seager’s orchedometer. MGG stain and NFI stain were done together with folw cytometric analysis of their semen. TESE and histopathological examination were also done. Results: A significant correlation was noticed between MGG stain and TESE results showing that among 14 patients who were spermatid positive for MGG, 14% were TESTE negative and 85.7% were found to be TESE 14.3% were TESE positive (positive predictive value of of MGG was 85%.71). no significant correlation could be detected between NFI stain results and TESE. As regards the correlation between haploid cells detected by folwcytometry and TESTE results it was found that of the 38 patients showing no haploid cells by flowcytometry, 76.3% were TESTE negative while 23.7% were TESTE positive. On the other hand 27 patients showing haploid cells by flowcytometry, 33.3% were TESTE negative while 66.7 were TESTE positive. There is a significant correlation between them. (Positive predictive value of flowcytometer was 66.67). When MGG stain and haploid cell detected by flowcytometer were correlated to TESTE results it showed that among the 11 patients who were MGG positive and haploid cells positive by flowcytometry 9.1% were TESTE negative while 90.9% were TESTE positive. On the contrary 20 patients who were MGG negative and haploid cells negative detected by flowcytometry 90% were TESTE negative and only 10% were TESTE positive. (Positive predictive value of MGG and flowcytometer was 90.91). Conclusion: the flowcytometery may have a low positive predictive value of 66.67% due to the presence of debris in semen, while MGG stain has a positive predictive value of 85.7%, but flowcytometer MGG when used together will raise the predictive value of TESTE to 90.91% . Further research is needed to improve the flowcytometrical analysis and its reliability as a method for the prediction of TESTE results. The method has to be modified by using different dyes and different calibration techniques to get the utmost accurate result from this machine.