Background: Androgenetic alopecia (AGA) is the most common hair loss disorder, affecting both men and women. The adjective androgenetic describes the two dominant causal factors, namely genetic susceptibility and androgens. Genetic variability in the androgen receptor gene (AR) is the cardinal prerequisite for the development of early-onset AGA. Recent studies reported elevated prevalence of hypertension among patients with AGA, and it has been proposed that both phenomena may be explained by the presence of hyperaldosteronism. Our study was undertaken to assess whether female patients with AGA have an elevated serum level of aldosterone, free testosterone and dehydroepiandrosterone sulfate to assess their possible role in the pathogenesis of the disease, also if they have higher level of androgen and aldosterone receptors in scalp biopsies, and if there is difference in the level of androgen and aldosterone receptors in involved and non-involved areas.Material and Methods: This study comprised 20 female patients of AGA and 20 of healthy control. All cases were subjected to complete history taking, clinical examination including degree of AGA according to Ludwig scale. Estimation of the serum level of aldosterone, free testosterone and dehydroepiandrosterone sulfate (DHEA-S) was done for patients and controls. Also, aldosterone and androgen receptors were detected in scalp biopsies from involved and non-involved areas.Results: Our results showed statistical significant difference between cases and controls as regards serum level of aldosterone (P value < 0.001), and serum level of free testosterone (P value < 0.019), but the serum level of dehydroepiandrosterone sulfate (DHEA-S) is higher in cases but with NO significant statistical difference (P value < 0.176). Our study also showed that aldosterone receptors level in scalp biopsies is higher in involved areas (cases) than controls with significant statistical difference (P value < 0.000), aldosterone receptors level in scalp biopsies is higher in non-involved areas (cases) than controls with significant statistical difference (P value < 0.001) and aldosterone receptors level in scalp biopsies is higher in involved areas (cases) than non-involved areas (cases) with significant statistical difference (P value < 0.001). It also showed that androgen receptors level in scalp biopsies is higher in involved areas (cases) than controls with significant statistical difference (P value < 0.001), androgen receptors level in scalp biopsies is higher in non-involved areas (cases) than controls with significant statistical difference (P value < 0.001) and androgen receptors level in scalp biopsies is higher in involved areas (cases) than non-involved areas (cases) with significant statistical difference (P value < 0.002).Conclusion: The present study demonstrated a significant increase in serum levels of aldosterone and free testosterone in female patients with AGA in comparisons to the controls. Also, it showed a significant increase in the level of androgen and aldosterone receptors in skin biopsies taken from involved areas in comparison to non-involved areas. These results verify the role of these hormones and their receptors in the pathogenesis of AGA and this may open new lines of treatment of such cases.