Among the many proteins produced in the endometrium, glycodelin has been suggested as the most promising indicator of endometrial maturation. However, the results are still conflicting. Fibroids less than 4 cm that are interstitial and not encroaching on the cavity are also an area of debate in the field of assisted reproduction. A study was done on 120 females undergoing in vitro fertilization using long pituitary down regulation protocol in The Egyptian Centre for fertility And IVF over an 18 months period. Blood and uterine flushing samples were collected from each patient at day 21 before starting pituitary down regulation. Glycodelin levels were assayed in serum and uterine flushing samples using (ELISA). Patients were divided into those who conceived and those who failed to conceive. The subgroup of twenty patients with fibroids was also analyzed separately.Mean glycodelin levels were found significantly lower in conception than in non conception cycles. The range of glycodelin was too wide to determine a specific threshold that would allow measurement of glycodelin to be used as a clinical tool Mean glycodelin levels in uterine flushings were significantly higher than in serum in both groups. Mean levels of glycodelin were non significantly higher in patients with fibroids.