Human papillomavirus (HPV) has been considered to be an etiologicalagent for anogenital cancers, such as cervical cancer and possibly a subsetof cancers of the aerodigestive tract. The aim of the study was to evaluatethe presence of human papillomavirus DNA in colorectal carcinoma. Thepresent study was carried out on 40 patients with hisopathologicallyconfirmed primary colorectal cancer. These samples were obtained aftersurgical resection. Two samples were collected from each patient: onesample from the tumor site and the other one from normally appearingcolorectal tissue. Detection of HPV- 16 and 18 was done using real- timePCR. HPV 16 was detected in only 1/40 (2.5%) tumor sample while alladjacent normal tissues were negative for it. HPV 18 was detected in 5/40(2.5%) of tumor samples and in 10/40 (25%) of adjacent normal tissue.Total HPV (16/18) detection results were 6/40 (15%) in tumor tissuesamples while they were detected in (10/40) 25% of normal tissuesamples. None of the studied cases was infected by both HPV 16 and 18simultaneously.