Background: Amoebiasis, infection with E. histolytica, is usually diagnosed clinically or using microscopically that cannot differentiate pathogenic E. histolytica from a morphologically identical but non-pathogenic strains.Objectives: The present study aimed to determine the prevalence of infections caused by E. histolytica and the non-pathogenic Entamoeba, using microscopical examination and two immunoassays; E. histolytica/E. dispar ICT kit and E.histolytica ELISA kit and to evaluate the diagnostic effectiveness of used tests. Also, to analyze associated variables as the estimated risk for E.histolytica infections.Material and methods: This study included 194 faecal samples collected from patients suffering diahroea and/or dysentery attending Beni-suef university hospital. Their related data were recorded. All samples were examined for Entamoeba complex microscopically and copro-antigen of the E. histolytica/E. dispar and E. histolytica using ICT kit and Tech lab E. histolytica II ELISA test, respectively.Resultes: Microscopic examination revealed Entamoeba complex trophozoites/cysts in 20.6% and 22.2% of samples prior and after faecal concentration, respectively. ICT revealed E. histolytica/E. dispar in 14.4% and ELISA detected E. histolytica in only 3.6% of samples. The ICT method had a sensitivity of 28.6% and specificity of 86.1%. Microscopy and ICT reported recovery of E. histolytica (sensitivity; 57.1% and 28.6%, respectively) with poor PPV (9.3% and 7.1%, respectively). There was slight agreement between ELISA test and both the microscopy and ICT and. Data analysis proved that the increase in the risk of E. histolytica were among cases who had mucus and blood in their stool relatively than those didn`t had.Conclusion: The obtained results clearly demonstrated that the prevalence of E. histolytica was low (3.6%) in studied individuals and was 14 times lower than non-pathoegenic amoebae. E. histolytica faecal ELISA assay for E. histolytica detection surpassed microscopy and E. histolytica/E. dispar ICT assay. Among the analyzed study variables, mucoid and bloody stool were positively associated with risk of E. histolytica infection. This has highlighted the need for practical non-microscopic detection methods that can differentiate between amoeba infections to avoid unnecessary and possibly harmful therapies and to determine the true prevalence and epidemiology of E. histolytica.