The aim of this work was to investigate the usefulness of the Dsysphonia Severity Index (DSI) as an easily applicable objective multiparametric measure in assessing the severity of dysphonia and to compare its objective results with the perceptual assessment of voice by GRBAS scale. Furthermore it was investigated whether the DSI can differentiate between different diagnosis groups of dysphonia. This study included 30 patients with dysphonia and 30 normal subjects. The DSI was measured in the dysphonia group as well as the control group. The DSI in patients was significantly lower than the control group. Also the DSI could differentiate significantly between the three perceptual grades of dysphonia which were divided according to grade score of GRBAS scale, while there was no significant difference in the DSI between the functional dysphonia group and MAPLs group. In addition there was a highly significant negative correlation between the DSI as an objective measure and the perceptual Grade of GRBAS scale as a subjective measure. The DSI is a useful objective measure in the assessment of dysphonia and in quantifying the different degrees of dysphonia as identified by the perceptual Grades of GRBAS scale.