In the present study, one hundred female patients in the child bearing period (age 18-45yrs) were selected from those attending the Obstetric and Gynecology outpatient clinics, in Kasr El Aini hospital, Cairo University. Females were complaining of vaginal discharge with either itching, burning sensation or both. Vaginal swabs were obtained from all female patients for examination by direct wet mount examination, Giemsa staining, Modified Diamond culture and latex agglutination test ( Kalon ) to detect the presence of Trichomonas vaginalis infection. The percentage of infection was 5cases (5%) by all tests done collectively. Out of 100 examined cases, latex agglutination test detected 5 positive cases, 3 of them were detected by culture, 1 were detected by Giemsa staining and one was detected by wet mount. This case was also positive with all other methods. The wet mount, Giemsa staining and Kalon latex test had sensitivities of 33.3%, 33.3% and 100% respectively while their specificities were 100%, 100% and 97.9% respectively.