Objectives:. To evaluate the diagnostic value of urinary trypsinogen-2 dipstick test for early diagnosis of post endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis.Methods: A total of 37 patients were tested with the urinary trypsinogen-2 dipstick test and serum levels of amylase and lipase before ERCP and 6 hours after ERCP. Results: Post-ERCP pancreatitis was diagnosed in 6 (16%) of 37 patients. The sensitivity, specificity, positive predictive value and negative predictive value of urinary trypsinogen-2 dipstick test at 6 hours after ERCP were100%, 97%, 86%, 100%,respectively. At the cutoff level (130 u/l) for lipase the positive predictive value and negative predictive value all were (100%) , however, the positive predictive value and negative predictive value for amylase levels at cutoff (122 u/l) were (60%, 100%) respectively.The lipase test was the best test for diagnosing post- ERCP pancreatitis followed by the urinary trypsinogen-2 dipstick test.Conclusions: The urinary trypsinogen-2 dipstick test can be used as a rapid and easy test for early diagnosis of post-ERCP pancreatitis with high sensitivity and specificity.