The present study was performed on 92 selected cases from breast cancercases received in National Cancer Institute, Kasr Al Aini UniversityHospital, and Tanta Cancer Center during the period from 2006 to 2010.The aim of the current study was to determine prostate-specific antigen(PSA) & androgen receptor (AR) in breast cancer cases using tissuemicroarray technique, and to assess their relation withclinicopathological features and hormone receptors status. It was foundthat PSA & AR were expressed in (70.65%) & (71.74%) of breast cancerstudied cases respectively. Both markers were significantly correlated.PSA had significant association with the size of the tumors, estrogenreceptor (ER), and human epidermal growth factor receptor type 2 (HER-2). AR was inversely correlated with ER and associated significantly withtriple negative cases. These findings may have clinical implications forthe treatment and prognosis, particularly of hormone negative,unresponsive hormone positive, and triple negative breast cancerpatients.