Carbapenems are a class of Beta-lactam antibiotics with broad spectrum of antibacterial activity. They are often considered as a last resort in treatment of infections caused by multidrug-resistant organisms. Carbapenemase-producing Enterobacteriaceae (CPE) have been reported worldwide.Resistance to carbapenems in Enterobacteriaceae is caused mainly by carbapenemase production or by porin loss combined with the expression of β-lactamases like an extended-spectrum Beta-lactamase (ESBL) or AmpC. Klebsiella pneumoniae carbapenemase (KPC) class A (serine carbapenemases is one of the most prevalent carbapenemases in Enterobacteriaceae. In the present study, we attempted to determine the prevalence of KPC among 100 clinical isolates of Enterobacteriaceae by phenotypic confirmatory tests; the modified Hodge test (MHT) and boronic acid inhibition test and to compare both methods by PCR for blaKPC gene Initial screening for carbapenemase-producing isolates among the 100 Enterobacteriaceae isolates was done using meropenem and ertapenem by disk diffusion method followed by MIC determination by E-test. Confirmation of KPC among carbapenem non-susceptible isolates was done by phenotypic methods as MHT and boronic acid inhibiton test and by genotypic methods as PCR. The Results of the current study showed that the prevalence of carbapenemase producing isolates by MHT was 51% and prevalence of KPC by carbapenem-boronic acid combined disk test was 35%, while it was 49% by PCR.