Introduction: Peripheral pulmonary lesions are increasing innumbers including; peripheral lung cancer, tuberculosis, pneumonias andothers. Pathological diagnosis is essential for prevention of unnecessaryoperation. Non-surgical methods for diagnosis of the nature of theselesions include; CT-guided transthoracic needle biopsy and endobronchialultrasound (EBUS).The Aim of the work is to assess and compare the role of CTguidedbiopsy and EBUS-guided biopsy in diagnosis of peripheral lungshadows. Subjects and methods: Thirty one cases were included in thepresent study; 22 males and 9 females who had peripheral pulmonaryshadows (radiological shadows with absence of endobronchialabnormality on conventional white light bronchoscopy). EBUS-guidedbiopsy was done for all cases and CT-guided biopsy was done only for 13cases. Results: It was found that EBUS-guided TBLB was more sensitivethan CT-guided biopsy and there was a significant difference as regardthe help in diagnosis of peripheral pulmonary shadows without detectedconsiderable complications. Conclusion: EBUS-guided biopsy is a feasible and safe method fordiagnosis of peripheral pulmonary lesions and it appears to be at leastequivalent to CT-guided biopsy without the accompanying radiationexposure.