This study demonstrated a good concordance between the depicted ultrasound retinal features and those of corresponding retinal histology. Visualization of such fine details (cytological level) and thin layers as histology requires very high frequency ultrasound (Giga-Hertz range) and was beyond the scope of the current study. The assignment of normal retina ultrasound signature with histology has been made possible combining the correlation between echo depth and the position of the layers on histologic sections, ultrasound and Histologic thickness of well resolved layers and echo- and histologic-structure of the layers. Ultrasound images of normal rat retina displayed four distinct sub-layers marked by echoic bands arising from reflection at interfaces of structure of different density and/or elasticity. These images are concordant with the four layered ultrasound biomicroscope appearance of peripheral retina from human dissected globes previously reported at 100 MHz.