Surgical sites infection(SSI) remain a major clinical problem in terms of morbidity, mortality, and hospital costs. The rate of surgical site infection and the frequency of various pathogens causing SSI with their antibiotic resistence pattern in surgical department of Beni-Suef University Hospital were studied. Methods: In the period from April to October 2009, 435 patients were admitted for surgery. 60 isolates of wound swabs were collected from patients who got SSI according to CDC definition of SSI. 20 out of 60 wound isolates were pseudomonas spp. Isolates of pseudomonas spp. From wound swabs and environmental samples were subjected to phenotyping using antibiogram and genotyping using RAPD-PCR.Results: The overall infection rate was 13.7%. pseudomonas spp. was the commonest isolated microorganism(33.33%) followed by staphylococcus aureus (16.7%).Coclusion:There was a similarity between clinical and environmental isolates but there was no association between antibiotype and genotype.