There is a constant need to improve the performance of current diagnostic assays of schistosomiasis as well as develop innovative testing strategies to meet new testing challenges. The use of nanoparticles as tags or labels allows for the detection of infectious agents in small volumes with a very sensitive, specific and at lower costs than current in-use technologies. This study aims at increasing the diagnostic efficiency of monoclonal antibody (MAb)-based antigen detection assays through nanoparticles conjugated with specific anti-Schistosoma mansoni monoclonal antibodies. In this study, several hybidoma cell lines secreting MAbs against adult worm tegumental Schistosoma antigen (AWTA) were produced at Immunology Department of TBRI and preserved in liquid nitrogen. One MAb (6D/6F) raised against adult worm tegumental Schistosoma antigen (AWTA) was chosen for this study due to its high reactivity to Schistosoma antigen as it gave highest optical density (OD) values. Gold nanoparticles were functionalized before their conjugation with MAb. The study was conducted on serum samples of 116 subjects. 71 patients with S. mansoni eggs in their stool samples group (gp 1) , 25 with other parasites ( gp2) and 20 negative healthy controls ( gp3). Patients in gp1 were further subdivided according to egg count in their stool samples into Light infection { ≤ 50 egg per gram(epg) (n= 17)}, moderate {51-100 epg (n= 33)} and severe infection { >100 epg(n= 21) }. Sandwich ELISA was performed using gold-MAb (AuNPs -MAb) for detection of CSA levels in serum samples of all groups and the results were compared with that after using MAb/sandwich ELISA system. Gold- MAb/ ELISA system reached a lower detection limit of 10 ng/ml compared to 85 ng/ml on using MAb/ ELISA and the optimal concentrations of gold-MAb were found to be 12 less than the optimal concentrations of MAb for detection of CSA.The sensitivity and specificity of sandwich ELISA for detection of CSA levels using gold-MAb were determined 100% and 97.8 % respectively compared to 87.3% sensitivity and 93.38 % specificity on using MAb/ ELISA system. It was found that CSA was detected in 9 out of 71 S. mansoni infected patients on using gold- MAb/ ELISA system and was not detected by MAb/ ELISA system. All those patients (9) were found to have an egg count below 50 epg feces (patients with light infections). ROC curve analyses revealed that sandwich ELISA using gold-MAb was an excellent diagnostic investigator that could differentiate Schistosoma patients from healthy controls with an AUC (the areas under the ROC curve), on the other hand sandwich ELISA using MAb was not accurate enough as it could not recognize nine out of 71 patients with light infections (1). Significant positive correlations were detected between ova count (intensity of infection) and OD reading on using gold-MAb Schistosoma infected group.