Nephrotoxicity has limited the clinical use of cisplatin in 25-30% of patients, even since the first dose. Although the exact mechanism of CP-induced nephrotoxicity is not well understood, several studies have suggested the involvement of oxidative stress in the development of this drug’s renal tubule injury.L-carnitine, through its potential possible antioxidant effect, would have a nephro- protective effect against cisplatin-induced nephrotoxicity.In this study, we intended to study the possible protective effect of l-carnitine (500 mg/kg I.P.) in experimentally induced nephrotoxicity by single bolus dose (5 mg/kg I.P.) and 5-day regimens (1 mg/kg for 5 days I.P.) of cisplatin, and to compare the extent of nephrotoxicity induced by the two regimens of cisplatin.We classified our mature male albino rats into eight groups: a control group, L-carnitine group, cisplatin single bolus dose, 5-day regimen of cisplatin, single dose of cisplatin with L-carnitine, 5-day regimen of cisplatin with L-carnitine, L-carnitine as prophylactic only with 5-day regimen of cisplatin and L-carnitine as treatment only with 5-day regimen of cisplatin. Cisplatin administration resulted in significant increased absolute and relative kidney weight, BUN, serum creatinine and MDA, significant decrease in animal body weight, GSH and SOD and Histopathological injury of the kidney. However single bolus dose of cisplatin was more toxic than 5-day regimen of cisplatin. L-Carnitine injection with cisplatin protected the kidney indicated by significant decreased absolute and relative kidney weight, BUN, serum creatinine and MDA, significant increased animal body weight, GSH and SOD and improvement of the kidney injury. However L-carnitine was more protective when given as both prophylactic and treatment than prophylactic only or treatment only.