The present study was conducted on 177 children, in the age group from 1 to 5 years old, suffering from diarrhea, selected from the Gastroenterology Unit Abu El Rish University Hospital for the detection of Cryptosporidium infection. Demographic, clinical and environmental related data were taken following an included sheet. A control group of 35 apparently healthy children in the same age group was included. Cases were screened using RIDA Quick Cryptosporidium coproantigen detection kit. In addition, stool specimens were examined following the Kinyoun acid-fast staining method. Results revealed that 27 children in the study group were positive for Cryptosporidium infection using antigen detection method (15.3%), while 20 were positive using the acid-fast technique (11.3%). All children in the control group were negative for Cryptosporidium infection.