Background :It is well established that genetic factors play an important role in the development of type 2 diabetes (T2D) and its chronic complications. Uncoupling protein 2 (UCP2) is expressed in several tissues, and acts in the protection against oxidative stress, some studies have reported the common polymorphisms in UCP2 gene -866G/A is possibly associated with cardiovascular complication of diabetic patients with inconclusive results. Myeloperoxidase (MPO) plays an important role in the initiation, progression, and the complications of atherosclerosis. It is one of the most promising biomarkers of oxidative stress for clinical cardiologists. Objective: The objective of this study was to evaluate the association between UCP2 gene, -866G/A polymorphism with cardiovascular disease and obesity in diabetic patients’ type2 and role of MPO in cardiovascular disease in diabetic patients, also the association between MPO and UCP2 gene polymorphism in diabetic patients. Subjects and Methods: In this study 40 diabetic patients complicated with CVD were compared with 40 patients with T2D without CVD complication. MPO was measured using ELISA method. The UCP2 gene -866G/A polymorphism were studied by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results: MPO was statistically significantly correlated with T.chol and LDL among all diabetic patients and in non-CVD group. There were negative correlation between MPO and HDL among all diabetic patients and non-CVD group but not statistically significant. There was no significant association between the UCP2 gene polymorphism -866G/A and cardiovascular complication of type 2 diabetic patients. HDL was lower in GG subjects as compared to GA/AA but did not reach statistical significance. There was no significant association between MPO and UCP2-866G/A gene polymorphism. Conclusion : Our findings provide further insight into the use of MPO to better predict the natural history of atherosclerosis and CVD in diabetic patients. Although the data in this study was not statistically significant, the A allele in the UCP2 gene promoter seems to be protective against obesity and Cardiovascular complication of T2D.