Premature rupture of the fetal membranes (PROM) is defined as the rupture of the fetal membranes with release of the amniotic fluid more than 1 hour prior to the onset of labor.PROM may be subdivided into term PROM (TPROM, i.e. PROM after 37 weeks of gestation) and preterm PROM (PPROM, i.e. PROM prior to 37 weeks of gestation). PPROM is responsible for a third of all preterm births.This study was performed on 60 pregnant women recruited from Cairo University (Kasr Al-Aini) Maternity Hospital’s outpatient clinic and casuality unit with gestational age more than 28 weeks and gave suspected history of ROM. They were divided into two groups according to the results of the vaginal fluid cytology.According to the results of this study, Amniosense sensitivity to detect PROM was 96.67%, specificity was 100% and accuracy was 98.33% while nitrazine test sensitivity was 83.33%, specificity was 90% and accuracy was 86.67%.