Coronary heart disease (CHD) develops in women on average 10 years later than in men. This lag has been attributed, to the protective effects of estrogens before menopause. Both natural and synthetic estrogens have antiinflammatory plus vasoprotective effects. 64 female albino rats were included in this study and classified into four main groups; each group included 16 rats. Group1 (Control group), group 2 immobilization group (IMO), group 3 IMO + ovarectomy (OVE), group 4 (IMO+ OVE + estrogen supplementation 10 µg/100 Kg, once daily for 2 weeks).Immobilization stress (IMO) group showed significant increase in systolic blood pressure (SBP) and diastolic blood pressure (DBP). Also there was significant increase in gene expression of c-FOS (functional marker of cellular activation) and heat shock protein (cardioprotective substance, HSP70) & significant decrease in contractility index in comparison to control group.Ovarectomized (OVE) + IMO group showed significant increase in SBP, DBP, c-FOS, and significant decrease in atrial naturetic peptide (ANP) & HSP70 in comparison to IMO group.Administration of estrogen to OVE rats caused a significant decrease in SBP, DBP, c-FOS and a significant increase in ANP & HSP70 after immobilization stress in comparison to IMO+ OVE group.In conclusion, estrogen supplementation therapy attenuates the cardiac changes that occur in ovarectomized rats after immobilization stress, taking into consideration the side effects of estrogen.