Rift Valley fever (RVF) is an acute, fever-causing viral disease that affects domestic animals (such as cattle, buffalo, sheep, goats, and camels) and humans. RVF is most commonly associated with mosquito-borne epidemics during years of heavy rainfall. The disease is caused by the RVF virus, a member of the genus Phlebovirus in the family Bunyaviridae. The disease was first reported among livestock by veterinary officers in Kenya in the early 1900s. This study was done to determine the prevalence of RVF virus in Egypt by screening IgM and IgG in a random sector of susceptible persons referred to the Central Public Health Laboratory of Ministry of Health (CPHL of MOH). The association of these antibodies to age, sex and occupation as well as regional prevalence is to be screened. This is to be compared to a control group of healthy individuals referred to (CPHL of MOH) for other check up reasons. In addition a group of other Arboviruses were IgG assayed , they are West Nile, Sandfly and Sindbis viruses, this was done to screen for the magnitude and endemicity of these viruses in relation to RVF virus.The study revealed that there was a mild positive correlation between RVF IgM and "AGE", RVF IgG and Age, the percentage of positivity of RVF IgG for male and female was 2:1 possibly related to occupation as male jobs are mostly related to animals as there was a positive correlation between RVF IgG and "Occupation", there was a mild positive correlation between governorate and IgM, meaning that patients in certain governorate had more chances to catch infection.There were 4 patients both RVF IgG +ve and IgM +ve, all from Dakahlia all are males all in middle age all related to animal jobs except one student who may be intimate to animals at home, there were also 3 patients RVF IgG –ve but IgM +ve, 2 from Dakahlia, males, one child of 3 years and one cattle rising of 9 years, the third was from Monofia, a 60 years old male farmer.A mild correlation was also found between West Nile IgG and "Governorates", Sandfly IgG and "Governorate distribution”. And a moderate correlation was found between West Nile IgG and "AGE", Sandfly IgG and "AGE".